Fourier microscopy is widely used for single molecule imaging, surface plasma observation, photonic crystal imaging, etc. It makes the direct observation of the spatial frequency distribution possible. The image quality of single molecule depends on the high-NA Fourier microscopy system, e.g. the angle-dependent Fresnel loss at each optical interfaces in the complex lens systems and the diffraction from the aperture. VirtualLab Fusion can model the entire system with the Fresnel loss and aperture diffraction effects considered. An example is presented in and we compare the simulation results with experimental results from literature.
